flow cytometry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
western blot: suitable
TREM-2, also known as Triggering receptor expressed on myeloid cells 2 or Triggering receptor expressed on monocytes 2, and encoded by the gene Trem2/Trem2a/Trem2b/Trem2c, is a protein that forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.
TREM-2 may also have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM-2 is localized to the cell membrane and is a single pass membrane protein.
TREM-2 is expressed in immune cells such as microglia and monocytes but the expression varies depending upon the exact tissue and location within the tissue. Defects in TREM-2 may be associated in humans with frontotemporal dementia, Nasu-Hakola disease, and recently an increase risk of Alzheimer′s disease.