Mouse Anti- (PVL LukS) mAb (1D9)

Catalog #:

0221-001

Immunogen:

Recombinant LukS-PV (His tag)

Description:

Mouse monoclonal antibody reactive to a conformational epitope of Staphylococcus aureus Panton Valentine Leukocidin LukS subunit.

Supplied:

100 µg is supplied in PBS at a concentration of 3.98

mg/mL.

Purification:

Antibody is purified using immobilized protein A.

Clonality:

Monoclonal of the IgG2b isotype.

Relevance:

The antibody can be used for detection of LukS-PV in an ELISA sandwich assay. It is not recommended for denatured Western blot detection due to the recognition of a partially conformational epitope of the LukS.

ELISA:

Assay-dependent dilution. Internal QC demonstrates good capture of purified recombinant LukS-PV using this monoclonal antibody coated to a plate at 100 ng/well.

WB:

It is not recommended to use this antibody for Western Blotting application due to weak detection of LukS-PV.

Cross Reactivity:

As with most antibodies, this antibody may interact with Protein A in S. aureus culture supernatant via the Fc region. Other cross reactive epitopes are not known at this time.

Storage:

2-3 weeks +4oC, -20◦C long term