indirect ELISA: 1:2,500 using FITC-conjugate indirect immunofluorescence: suitable
isotype
IgG1
shipped in
dry ice
General description
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well defined hapten.
Specificity
The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).
Mouse monoclonal clone FL-D6 anti-FITC antibody will react with either free or conjugated FITC. The antibody does not react with bound or free TRITC (tetramethylrhodamine isothiocyanate).
Immunogen
FITC-BSA conjugate
Application
Mouse monoclonal clone FL-D6 anti-FITC antibody may be used for the detection of FITC and as a universal indicator reagent for bridging FITC with other immunochemical reagents. It may be used in ELISA, competitive ELISA and immunofluorescent techniques. A FITC Anti-FITC system has been used in the amplification of signal in immunofluorescent detection and as a means of separating bound from free tracer by affinity chromatography. The antibody can also be used to isolate cells that have an FITC-labeled ligand on their surface.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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